The Matlab code I wrote to compute the speckle modulation for the publication:

Mike Adams, Alex Matov, Daphne Yarar, Steph Gupton, Gaudy Danuser, Clare Waterman "Signal Analysis of Total Internal Reflection Fluorescent Speckle Microscopy and Wide-Field Epi-Fluorescence FSM of the Actin Cytoskeleton and Focal Adhesions in Living Cells (2004)"

https://researchgate.net/publication/8204587_Signal_analysis_of_total_internal_reflection_fluorescent_speckle_microscopy_TIR-FSM_and_wide-field_epi-fluorescence_FSM_of_the_actin_cytoskeleton_and_focal_adhesions_in_living_cells

My software provides the number of speckles in a ROI and their mean modulation; getModulation.m is the main function